Intro NMR Workshop - Oct. 10 and 11, 2018 - 9-12 each day

2 DAY Workshop: Introduction to Practical Aspects of Solution NMR for Chemists

Wednesday Oct 10 to Thursday Oct 11: 9 am to noon each day

This introductory workshop is geared toward undergraduate students, and new graduate students with limited NMR experience, but who are expected to make use of the NMR spectrometers as part of their research projects.

The workshop is meant as a supplement to the "one on one" training that new NMR users receive and will allow an opportunity to go into greater depth on many topics, therefore students who have recently started to use our NMR instruments are especially encouraged to attend. There will be an Advanced NMR Workshop offered next in the summer.

The schedule will consist of a lecture to start each day followed by hands-on learning sessions where participants will get the opportunity to collect and process NMR data.

The topics covered include:

• How an NMR spectrometer works
• NMR Magnet Safety
• Preparation of NMR Samples
• Set up of 1D 1H and 13C NMR Experiments
• Processing and Presentation of NMR Data

The workshop is free of charge to members of the Dept. of Chemistry and Biochemistry; however, enrollment is limited, so please register soon!

To register use the following link:

http://www1.uwindsor.ca/chemistry/nmr/chemistry/nmr/39/nmr-workshops

NMR Workshops | Nuclear Magnetic Resonance Facility - University of Windsor

www1.uwindsor.ca

2 DAY Workshop: Introduction to Practical Aspects of Solution NMR for Chemists   Wednesday Oct 10 to Thursday Oct 11: 9 am to noon each day    

NMR Workshop - May 23 and May 24

2 DAY Workshop: Introduction to Practical Aspects of Solution NMR for Chemists
Wednesday May 23 to Thursday Thursday 24 : 9 am to noon each day

This introductory workshop is geared toward undergraduate students, and new graduate students with limited NMR experience, but who are expected to make use of the NMR spectrometers as part of their research projects.

The workshop is meant as a supplement to the "one on one" training that new NMR users receive and will allow an opportunity to go into greater depth on many topics, therefore students who have recently started to use our NMR instruments are especially encouraged to attend. There will be an Advanced NMR Workshop offered later in the summer.

The schedule will consist of a lecture to start each day followed by hands-on learning sessions where participants will get the opportunity to collect and process NMR data.

The topics covered include:

• How an NMR spectrometer works
• NMR Magnet Safety
• Preparation of NMR Samples
• Set up of 1D 1H and 13C NMR Experiments
• Processing and Presentation of NMR Data

The workshop is free of charge to members of the Dept. of Chemistry and Biochemistry; however, enrollment is limited, so please register soon!

Please note that a separate Intro Workshop will be held for MMB students in July so MMB students should not register for this workshop.

To register click here

For more information, contact:

Matthew Revington
NMR Facility Manager, RM 394-5 Essex Hall
Department of Chemistry and Biochemistry
University of Windsor
519-253-3000 Ext 3997

Seminar: Leah Casabianca (Clemson University) - Friday, Jan. 26, 2018 @ 3:00 p.m.

Seminar: Leah Casabianca (Clemson University)

UWinChemBiochem Seminar Series - Winter 2018

Leah Casabianca
Department of Chemistry
Clemson University, Clemson, SC  
Title: “Characterization of Nanoparticle Surface Interactions Using Dissolution Dynamic Nuclear Polarization and Saturation-Transfer Difference NMR”
Web: https://chemistry.sites.clemson.edu/casabiancagroup/

Friday, Jan. 26, 2018 @ 3:00 p.m.
Room #186 Essex Hall

**Everyone Welcome**

Characterization of Nanoparticle Surface Interactions Using Dissolution Dynamic Nuclear Polarization and Saturation-Transfer Difference NMR

Leah B. Casabianca, Department of Chemistry, Clemson University

Nanotechnology is becoming increasingly prevalent in our everyday lives. Nanoparticles that are used as lubricants, in drug delivery, and as antibacterial agents are finding their way into the body and into the environment, where they interact with biological macromolecules such as proteins. Understanding the nature of the interactions between nanoparticles and adsorbed molecules is therefore increasingly relevant in fields such as drug delivery, nanoparticle catalysis, and nanoparticle toxicity. In this talk, I will discuss several recent studies in my lab that are aimed at developing Nuclear Magnetic Resonance (NMR) techniques for studying noncovalent nanoparticle surface interactions.

NMR is an incredibly powerful characterization technique, capable of providing atomic-level structural as well as dynamic information. However, NMR is not ideally suited for surface studies due to the inherent low sensitivity of this technique. One way of improving the sensitivity of NMR is Dynamic Nuclear Polarization (DNP). DNP relies on the transfer of polarization from a nearby unpaired electron to nuclei of interest in NMR. My group has recently¹ developed HighlY-effective Polymer/Radical Beads (HYPR-Beads), which are organic nanoparticles that have been doped with radicals for use as DNP polarization agents. Using HYPR-beads, we were able to hyperpolarize nuclei in small molecules that are located near the beads in an aqueous environment.

We are also using Saturation Transfer Difference (STD)-NMR Spectroscopy to identify small molecules that interact noncovalently with the surface of functionalized organic nanoparticles in solution.^2,3 STD-NMR was originally developed to identify small-molecule ligands that bind to a particular protein receptor. Since this technique does not require the receptor to be seen by solution-state NMR, there is no upper limit to the size of the receptor that can be studied. This makes the STD-NMR technique an ideal one to study small molecules adsorbed on the surface of nanoparticles. We have used STD-NMR to determine the binding constant between small molecules and solvent water on the surface of nanoparticles,² and to determine the binding epitopes of a fluorescent dye associating with the nanoparticle surface.³ This work has future applications in determining the structure of proteins adsorbed on the surface of nanoparticles, and in the development of dual-use imaging contrast agents.

References

[1] Y. Zhang, P. J. Baker, L. B. Casabianca, “BDPA-Doped Polystyrene Beads as Polarization Agents for DNP-NMR.” J. Phys. Chem. B 2016, 120, 18-24.

[2] Y. Zhang, H. Xu, A. M. Parsons, L. B. Casabianca, “Examining Binding to Nanoparticle Surfaces Using Saturation Transfer Difference (STD)-NMR Spectroscopy.” J. Phys. Chem. C 2017, 121, 24678-24686.

[3] Y. Zhang, H. Xu, L. B. Casabianca, “Interaction Between Cyanine Dye IR-783 and Polystyrene Nanoparticles in Solution.” submitted.

Bio: Dr. Casabianca received a B. S. degree in chemistry from Rice University in 2002 where she did undergraduate research with Prof. Seiichi P.T. Matsuda.  She completed her Ph. D. in the lab of Prof. Angel C. de Dios at Georgetown University in 2008.  Her thesis work involved using solution-state NMR and chemical shift calculations to understand the mechanism of action of anti-malarial drugs.  She then did a postdoctoral fellowship (2008-2010) with Prof. Yoshitaka Ishii at the University of Illinois at Chicago working on structural characterization of graphite oxide.  Her second postdoctoral fellowship was in the group of Prof. Lucio Frydman at the Weizmann Institute (2010-2013) where she worked on dynamic nuclear polarization of diamond samples.  She joined the faculty at Clemson University in 2014.

2 DAY Workshop: Introduction to Practical Aspects of Solution NMR for Chemists - Oct. 11 and 12

2 DAY Workshop: Introduction to Practical Aspects of Solution NMR for Chemists - Oct. 11 and 12

2 DAY Workshop: Introduction to Practical Aspects of Solution NMR for Chemists

Wednesday October 11 to Thursday October 12 : 9 am to noon each day

This introductory workshop is geared toward undergraduate students, and new graduate students with limited NMR experience, but who are expected to make use of the NMR spectrometers as part of their research projects.

The workshop is meant as a supplement to the "one on one" training that new NMR users receive and will allow an opportunity to go into greater depth on many topics, therefore students who have recently started to use our NMR instruments are especially encouraged to attend. There will be an Advanced NMR Workshop offered later in the summer.

The schedule will consist of a lecture to start each day followed by hands-on learning sessions where participants will get the opportunity to collect and process NMR data.

The topics covered include:

• How an NMR spectrometer works
• NMR Magnet Safety
• Preparation of NMR Samples
• Set up of 1D 1H and 13C NMR Experiments
• Processing and Presentation of NMR Data

The workshop is free of charge to members of the Dept. of Chemistry and Biochemistry; however, enrollment is limited, so please register soon!

Please note that a separate Intro Workshop will be held for MMB students in July so MMB students should not register for this workshop.

To register click here

Advanced NMR Workshop - June 21 and 22

Advanced NMR Workshop
Advanced Techniques, 2D NMR, and Introduction to Solid State NMR
Wednesday June 21 and Thursday June 22, 9am to noon each day

The workshop will briefly cover the physical theory behind the advanced NMR experiments but the focus will be on demonstrating the uses of these experiments and practical aspects of experimental set up.  The schedule will consist of a mixture of lectures and of hands-on sessions on the NMR spectrometers where participants will get the opportunity to set up experiments and collect data.

The topics covered include topics covered include:

• DOSY

• COSY
• NOESY/ROESY

• HMQC/HMBC

• Differences between solution and solid state NMR
• Solid State NMR sample preparation
• Magic-angle spinning – MAS  (tuning the angle with KBr)

• Cross-polarization (CP) MAS
• Basic 1H and 13C SSNMR experiments
• Pulse field gradients
• Shaped Pulses

• Selective 1D NOESY
• HOMODEC

• DEPTQ
• PSYCHE (broadband homonuclear decoupling)





This advanced workshop is available to undergraduate, graduate students and postdocs who have already taken the Introductory NMR Workshop or who have completed the third year Spectroscopic Structure Identification course (59-330/332) (or equivalent) and who have demonstrated sufficient competence to the NMR Facility Manager. No experience in solid state NMR is required.

Enrolment is free but numbers are limited.



Registration can be done using the link below or at the Department of Chemistry and Biochemistry-NMR Facility webpage

To register click here

For more information, contact: 
Matthew Revington
NMR Facility Manager,
Department of Chemistry and Biochemistry
University of Windsor
519-253-3000 Ext 3997
http://scholar.google.ca/citations?hl=en&user=YWRVOsUAAAAJ 

2 Day Workshop: Introduction to Practical Aspects of Solution NMR for Chemists

2 Day Workshop: Introduction to Practical Aspects of Solution NMR for Chemists

Wednesday May 24 and Thursday May 25: 
9:00 am to 12:00 pm each day

This introductory workshop is geared toward undergraduate students, and new graduate students with limited NMR experience, but who are expected to make use of the NMR spectrometers as part of their research projects.

The workshop is meant as a supplement to the "one on one" training that new NMR users receive and will allow an opportunity to go into greater depth on many topics, therefore students who have recently started to use our NMR instruments are especially encouraged to attend. There will be an Advanced NMR Workshop offered later in the summer.

The schedule will consist of a lecture to start each day followed by hands-on learning sessions where participants will get the opportunity to collect and process NMR data.

The topics covered include:

• How an NMR spectrometer works
• NMR Magnet Safety
• Preparation of NMR Samples
• Set up of 1D 1H and 13C NMR Experiments
• Processing and Presentation of NMR Data

The workshop is free of charge to members of the Dept. of Chemistry and Biochemistry; however, enrolment is limited, so please register soon!

Please note that a separate Intro Workshop will be held for MMB students in July so MMB students should not register for this workshop.

To register click here

NMR Workshop on Solution NMR - Oct. 13 and 14, 2016

2 DAY Workshop: Introduction to Practical Aspects of Solution NMR for Chemists 

Thursday October 13th and Friday October 14th: 9 am to noon each day 

This introductory workshop is geared toward undergraduate students, 410 students and new graduate students with limited (or no) NMR experience, but who are expected to make use of the NMR spectrometers as part of their research projects. 

The workshop is meant as a supplement to the "one on one" training that new NMR users receive and will allow an opportunity to go into greater depth on many topics, therefore students who have recently started to use our NMR instruments are especially encouraged to attend. Please inform new students in your lab who may not be on this mailing list.

The schedule will consist of a lecture to start each day followed by hands-on learning sessions where participants will get the opportunity to collect and process NMR data.

The topics covered include:

• How an NMR spectrometer works
• NMR Magnet Safety
• Preparation of NMR Samples
• Set up of 1D 1H and 13C NMR Experiments
• Processing and Presentation of NMR Data

The workshop is free of charge to members of the Dept. of Chemistry and Biochemistry; however,
enrolment is limited, so please register soon! 

To register click here

Matthew Revington
NMR Facility Manager
Department of Chemistry and Biochemistry
University of Windsor
519-253-3000 Ext 3997
http://scholar.google.ca/citations?hl=en&user=YWRVOsUAAAAJ

Advanced NMR Workshop - June 15-16, 2016

Advanced NMR Workshop 

Wednesday June 15 and Thursday June 16, 9am to noon each day

This advanced workshop is available to undergraduate students and/or graduate students/postdocs who have already taken the Introductory NMR Workshop or who have completed the third year Spectroscopic Structure Identification course (59-330/332) and who have demonstrated sufficient competence to the NMR Facility Manager. No experience in solid state NMR is required.

The workshop will briefly cover the physical theory behind the advanced NMR experiments but the focus will be on demonstrating the uses of these experiments and practical aspects of experimental set up .The schedule will consist of a mixture of lectures and of hands-on sessions on the NMR spectrometers where participants will get the opportunity to set up experiments and collect data.

The topics covered include topics covered include:

• DOSY

• COSY
• 
NOESY/ROESY

• HMQC/HMBC

• Differences between solution and solid state NMR
• Solid State NMR sample preparation
• Magic-angle spinning – MAS  (tuning the angle with KBr)

• Cross-polarization (CP) MAS
• Basic 1H and 13C SSNMR experiments
• 
Pulse field gradients
• Shaped Pulses

• Selective 1D NOESY
• HOMODEC

• DEPTQ





Enrolment is free but numbers are limited.



Registration can be done using the link below or at the Department of Chemistry and Biochemistry-NMR Facility webpage

To register click here

Matthew Revington
NMR Facility Manager
Department of Chemistry and Biochemistry
University of Windsor

2 DAY Workshop: Introduction to Practical Aspects of Solution NMR for Chemists

2 DAY  Workshop: Introduction to Practical Aspects of Solution NMR for Chemists

Date: Wednesday Oct 14 and Thursday Oct 15

Time: 9:00 am to 12:00 pm each day

Location: Science Resource Centre Rm 182 EH

This introductory workshop is geared toward undergraduate students, summer students and/or graduate students with limited (or no) NMR experience, but who are expected to make use of the NMR spectrometers as part of their research projects. 

The workshop is meant as a supplement to the "one on one" training that new NMR users receive and will allow an opportunity to go into greater depth on many topics, therefore students who have recently started to use our NMR instruments are especially encouraged to attend.   Please inform new students in your lab who may not be on this mailing list.

The schedule will consist of a lecture to start each day followed by hands-on learning sessions where participants will get the opportunity to make NMR samples as well as to collect and process NMR data.

The topics covered include:

• How an NMR spectrometer works
• NMR Magnet Safety
• Preparation of NMR Samples
• Set up of 1D 1H and 13C NMR Experiments
• Processing and Presentation of NMR Data

The workshop is free of charge to members of the Dept. of Chemistry and Biochemistry; however, enrollment is limited, so please register soon!

To register click here

Advanced NMR Workshop June 10 and 11

Announcement Advanced NMR Workshop: June 10 and 11

To register click here

With the fume hoods down do you have nothing fun to do this week, how about some NMR:

Advanced NMR Workshop

Wednesday June 10 and Thursday June 11, 9am to noon each day

This advanced workshop is available to undergraduate students and/or graduate students/postdocs who have already taken the Introductory NMR Workshop or who have completed the third year Spectroscopic Structure Identification course (59-330/332) and who have demonstrated sufficient competence to the NMR Facility Manager. No experience in solid state NMR is required.


The workshop will briefly cover the physical theory behind the advanced NMR experiments but the focus will be on demonstrating the uses of these experiments and practical aspects of experimental set up .

The schedule will consist of a mixture of lectures and of hands-on sessions on the NMR spectrometers where participants will get the opportunity to set up experiments and collect data.

 The topics covered include:

• DOSY
 
• COSY 
• 
NOESY/ROESY
 
• HMQC/HMBC
 
• Differences between solution and solid state NMR
 
• Solid State NMR sample preparation
 
• Magic-angle spinning – MAS (tuning the angle with KBr)
 
• Cross-polarization (CP) MAS
 
• Basic 1H and 13C SSNMR experiments 
• 
Pulse field gradients
 
• Shaped Pulses
 
• Selective 1D NOESY
 
• HOMODEC
 
• DEPTQ



 

Enrolment is free but numbers are limited.



Registration can be done using the link below or at the Department of Chemistry and Biochemistry-NMR Facility webpage

http://www1.uwindsor.ca/chemistry/nmr/